Wuhan Feiyue Biotechnology Co., LTD

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human A beta1-40. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human A beta1-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human A beta1-40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human A beta1-40. The concentration of Human A beta1-40 in samples can be calculated by comparing the OD of the samples to the standard curve.

Format:96T

Assay time:4.5h

Reactivity:Human

Detection Method:Colormetric

Detection Range:15.63-1000 pg/mL

Sensitivity:9.38 pg/mL

Sample Volume Required Per Well:100µL

Sample Type:Serum, plasma and other biological fluids

Specificity:This kit recognizes Human A beta1-40 in samples. No significant cross-reactivity or interference between Human A beta1-40 and analogues was observed.