Wuhan Feiyue Biotechnology Co., LTD

Feiyue's Human 3MH(3-methylhistidine) ELISA Kit (Human Activated Leukocyte Cell Adhesion Molecule Enzyme-Linked ImmunoSorbent Assay Kit) is an ELISA reagent for detection of 3MH(3-methylhistidine) in human serum, plasma or cell with sensitivity, specificity and consistency.

3-Methylhistidine (3-MH) is a post-translationally modified amino acid which is excreted in human urine.Urinary concentration of 3-methylhistidine is a biomarker for skeletal muscle protein breakdown in humans who have been subject to muscle injury.Urinary 3-methylhistidine concentrations are also elevated from consumption of soy-based products and meat, particularly chicken.

 This kit uses sandwich ELISA to detect the concentration of 3MH(3-methylhistidine).
3MH(3-methylhistidine)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized
antibody. A sandwich complex is formed by additional anti-human 3MH(3-methylhistidine) antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of human 3MH(3-methylhistidine) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

1."3-Methylhistidine". HMDB Version 4.0. Human Metabolome Database. 20 December 2017. Retrieved 25 December 2017.

2."3-Methyl-L-histidine". PubChem Compound. United States National Library of Medicine – National Center for Biotechnology Information. 23 December 2017. Retrieved 25 December 2017.

3.Chinkes DL (September 2005). "Methods for measuring tissue protein breakdown rate in vivo". Current Opinion in Clinical Nutrition and Metabolic Care. 8 (5): 534–537. doi:10.1097/01.mco.0000170754.25372.37. PMID 16079625. S2CID 8318490.

4.Holm L, Kjaer M (September 2010). "Measuring protein breakdown rate in individual proteins in vivo". Current Opinion in Clinical Nutrition and Metabolic Care. 13 (5): 526–531. doi:10.1097/MCO.0b013e32833c3c64. PMC 3008417.

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Q1. Is sample order available?
A: Yes, we welcome sample order to test and check quality.
Q2. What about the lead time?
A: Sample order: 3-5 days, mass order:10-12 days. The actual time depends on the order quantity.
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