Wuhan Feiyue Biotechnology Co., LTD

Feiyue's Human 5-HT(5-hydroxytryptamine) ELISA Kit is an ELISA reagent for detection of 5-HT(5-hydroxytryptamine), plasma or cell with sensitivity, specificity and consistency.

5-HT receptors, 5-hydroxytryptamine receptors, or serotonin receptors, are a group of G protein-coupled receptor and ligand-gated ion channels found in the central and peripheral nervous systems.They mediate both excitatory and inhibitory neurotransmission. The serotonin receptors are activated by the neurotransmitter serotonin, which acts as their natural ligand.The serotonin receptors modulate the release of many neurotransmitters, including glutamate, GABA, dopamine, epinephrine / norepinephrine, and acetylcholine, as well as many hormones, including oxytocin, prolactin, vasopressin, cortisol, corticotropin, and substance P, among others. Serotonin receptors influence various biological and neurological processes such as aggression, anxiety, appetite, cognition, learning, memory, mood, nausea, sleep, and thermoregulation. They are the target of a variety of pharmaceutical and recreational drugs, including many antidepressants, antipsychotics, anorectics, antiemetics, gastroprokinetic agents, antimigraine agents, hallucinogens, and entactogens.

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. T he concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve

Reference:

1.Hoyer D, Clarke DE, Fozard JR, Hartig PR, Martin GR, Mylecharane EJ, Saxena PR, Humphrey PP (1994). "International Union of Pharmacology classification of receptors for 5-hydroxytryptamine (Serotonin)". Pharmacol. Rev. 46 (2):  157–203. PMID 7938165.

2.Frazer A, Hensler JG (1999). "Chapter 13: Serotonin Receptors". In Siegel GJ, Agranoff BW, Albers RW, Fisher SK, Uhler MD (eds.). Basic Neurochemistry: MolecularCellular, and Medical Aspects. Philadelphia: Lippincott-Raven. pp. 263–292. ISBN 978-0-397-51820-

3.Beliveau, Vincent; Ganz, Melanie; Feng, Ling; Ozenne, Brice; Højgaard, Liselotte; Fisher, Patrick M.; Svarer, Claus; Greve, Douglas N.; Knudsen, Gitte M. (2017-01-04). "A High-Resolution In Vivo Atlas of the Human Brain's Serotonin System". Journal of Neuroscience. 37 (1): 120–128. doi:10.1523/jneurosci.2830-16.2016. PMC 5214625. PMID 28053035.

4.Nichols DE, Nichols CD (May 2008). "Serotonin receptors". Chem. Rev. 108 (5): 1614–41. doi:10.1021/cr078224o. PMID 18476671.

5. Murakami H, Murakami S (August 2007). "Serotonin receptors antagonistically modulate Caenorhabditis elegans longevity". Aging Cell. 6 (4): 483–8. doi:10.1111/j.1474-9726.2007.00303.x. PMID 17559503. S2CID 8345654.

FAQ:

Q1. Is sample order available?
A: Yes, we welcome sample order to test and check quality.
Q2. What about the lead time?
A: Sample order: 3-5 days, mass order:10-12 days. The actual time depends on the order quantity.
Q3. Do you have any MOQ limit for led light order?
A: Low MOQ, 1pc for sample checking is available
Q4. How do you ship the goods and how many days will it take?
A: Our main shipping agent are DHL, FedEx, UPS and TNT, etc. It takes 3-7 days.
Q5. Is it OK to use my own package?
A: Yes. Normally we offer white inner box and carton box. Your own design is ok.

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