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Human AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit Basic Information
Product Name | Human AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit |
Catalog NO. | FY-EH3547 |
Alias | AMPK ELISA Kit |
Detection Method | Sandwich ELISA, Double Antibody |
Application
| AMPK ELISA Kit allows for the in vitro quantitative determination of AMPK concentrations in serum, plasma, tissue homogenates and other biological fluids.
|
Size | 48T, 96T |
Storage | 2-8 ℃ for 6 months |
Sensitivity | < 0.188ng/ml |
Species | Human |
UniProt ID
| Q13131
|
CV %() | Intra-Assay: CV<8% Inter-Assay: CV<10% |
Note | For Research Use Only |
Feiyue's Human AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit is an ELISA reagent for detection of AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase), plasma or cell with sensitivity, specificity and consistency.
AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) IntroductionAMPK may stand for:AMP-activated protein kinase, an enzyme (acetyl-CoA carboxylase) kinase, an enzyme
Human AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit Test method This kit uses sandwich ELISA to detect the concentration of AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase). AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
Reference:
1.Suter M, Riek U, Tuerk R, Schlattner U, Wallimann T, Neumann D (October 2006). "Dissecting the role of 5'-AMP for allosteric stimulation, activation, and deactivation of AMP-activated protein kinase". The Journal of Biological Chemistry. 281 (43): 32207–16. doi:10.1074/jbc.M606357200. PMID 16943194.
2. Winder WW, Hardie DG (July 1999). "AMP-activated protein kinase, a metabolic master switch: possible roles in type 2 diabetes". The American Journal of Physiology. 277 (1): E1-10. doi:10.1152/ajpendo.1999.277.1.E1. PMID 10409121.
3. Hallows KR, Alzamora R, Li H, Gong F, Smolak C, Neumann D, Pastor-Soler NM (April 2009). "AMP-activated protein kinase inhibits alkaline pH- and PKA-induced apical vacuolar H+-ATPase accumulation in epididymal clear cells". American Journal of Physiology. Cell Physiology. 296 (4): C672-81. doi:10.1152/ajpcell.00004.2009. PMC 2670645. PMID 19211918.
FAQ:
Q1. Is sample order available?
A: Yes, we welcome sample order to test and check quality.
Q2. What about the lead time?
A: Sample order: 3-5 days, mass order:10-12 days. The actual time depends on the order quantity.
Q3. Do you have any MOQ limit for led light order?
A: Low MOQ, 1pc for sample checking is available
Q4. How do you ship the goods and how many days will it take?
A: Our main shipping agent are DHL, FedEx, UPS and TNT, etc. It takes 3-7 days.
Q5. Is it OK to use my own package?
A: Yes. Normally we offer white inner box and carton box. Your own design is ok.
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