Wuhan Feiyue Biotechnology Co., LTD

Feiyue's Human TIMP-3(Metalloproteinase inhibitor 3) ELISA Kit is an ELISA reagent for detection of SP(Alpha Crosslaps) in Mouse serum, plasma or cell with sensitivity, specificity and consistency.

Metalloproteinase inhibitor 3 is a protein that in humans is encoded by the TIMP3 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy.

Human TIMP-3(Metalloproteinase inhibitor 3) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of TIMP-3(Metalloproteinase inhibitor 3) The microtiter plate provided in this kit has been pre-coated with an antibody specific to TIMP-3(Metalloproteinase inhibitor 3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TIMP-3(Metalloproteinase inhibitor 3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain of TIMP-3(Metalloproteinase inhibitor 3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TIMP-3(Metalloproteinase inhibitor 3) in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

1,"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

2, "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

3, Apte SS, Mattei MG, Olsen BR (Jun 1994). "Cloning of the cDNA encoding human tissue inhibitor of metalloproteinases-3 (TIMP-3) and mapping of the TIMP3 gene to chromosome 22". Genomics. 19 (1): 86–90. doi:10.1006/geno.1994.1016. PMID 8188246.

FAQ:

Q1. Is sample order available?
A: Yes, we welcome sample order to test and check quality.
Q2. What about the lead time?
A: Sample order: 3-5 days, mass order:10-12 days. The actual time depends on the order quantity.
Q3. Do you have any MOQ limit for led light order?
A: Low MOQ, 1pc for sample checking is available
Q4. How do you ship the goods and how many days will it take?
A: Our main shipping agent are DHL, FedEx, UPS and TNT, etc. It takes 3-7 days.
Q5. Is it OK to use my own package?
A: Yes. Normally we offer white inner box and carton box. Your own design is ok.

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