Wuhan Feiyue Biotechnology Co., LTD

Feiyue's Human TATC(Thrombin-antithrombin complex) ELISA Kit is an ELISA reagent for detection of TATC(Thrombin-antithrombin complex) in Human serum, plasma or cell with sensitivity, specificity and consistency.

Thrombin–antithrombin complex (TAT) is a protein complex of thrombin and antithrombin.

TAT is formed in response to the high thrombin level caused by coagulation following a ruptured vessel. Since thrombin is rapidly bound by antithrombin, TAT is a good measure for thrombin level in the blood. Thrombin can pass the blood-brain barrier, destroying neurons and potentially causing cerebral edemas. TAT levels were studied in patients with intracranial blood clot removal within 24 hours after intracerebral hemorrhage (ICH) in Fujian from 2006–2008. This study revealed that TAT levels in the plasma and hematoma fluid of these patients are higher than that those of healthy people, and that TAT levels decreased in the patients after surgery and increased in the patients that had a hemorrhage again. The TAT levels correlate with the severity of ICH according to GCS and NIHSS, and so, the study concluded that TAT complex may be useful in the prognosis for post-operative ICH-patients.

Human TATC(Thrombin-antithrombin complex) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of  TATC(Thrombin-antithrombin complex) The microtiter plate provided in this kit has been pre-coated with an antibody specific to TATC(Thrombin-antithrombin complex). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TATC(Thrombin-antithrombin complex). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain of TATC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TATC(Thrombin-antithrombin complex) in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

1, Lippi G, Cervellin G, Franchini M, Favaloro EJ (2010). "Biochemical markers for the diagnosis of venous thromboembolism: the past, present and future". J. Thromb. Thrombolysis. 30 (4): 459–71. doi:10.1007/s11239-010-0460-x. PMID 20213258.

2, Zwicker JI, Furie BC, Furie B (2007). "Cancer-associated thrombosis". Crit. Rev. Oncol. Hematol. 62 (2): 126–36. doi:10.1016/j.critrevonc.2007.01.001. PMID 17293122.

FAQ:

Q1. Is sample order available?
A: Yes, we welcome sample order to test and check quality.
Q2. What about the lead time?
A: Sample order: 3-5 days, mass order:10-12 days. The actual time depends on the order quantity.
Q3. Do you have any MOQ limit for led light order?
A: Low MOQ, 1pc for sample checking is available
Q4. How do you ship the goods and how many days will it take?
A: Our main shipping agent are DHL, FedEx, UPS and TNT, etc. It takes 3-7 days.
Q5. Is it OK to use my own package?
A: Yes. Normally we offer white inner box and carton box. Your own design is ok.

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