Wuhan Feiyue Biotechnology Co., LTD

Feiyue's Human SORT1(Sortilin) ELISA Kit is an ELISA reagent for detection of SORT1(Alpha Crosslaps) in Mouse serum, plasma or cell with sensitivity, specificity and consistency.

Sortilin (SORT1) is a protein that in humans is encoded by the SORT1 gene on chromosome 1. This protein is a type I membrane glycoprotein in the vacuolar protein sorting 10 protein (Vps10p) family of sorting receptors. While it is ubiquitously expressed in many tissues, sortilin is most abundant in the central nervous system. At the cellular level, sortilin functions in protein transport between the Golgi apparatus, endosome, lysosome, and plasma membrane, leading to its involvement in multiple biological processes such as glucose and lipid metabolism as well as neural development and cell death.  Moreover, the function and role of sortilin is now emerging in several major human diseases such as atherosclerosis and coronary artery disease, Alzheimer’s disease, and cancer. The SORT1 gene also contains one of 27 loci associated with increased risk of coronary artery disease.

Given its function in facilitating lysosomal degradation or recycling of ligands in lipid metabolism and the neural system, sortilin likely plays an important role in the underlying mechanisms and pathophysiology of atherogenesis and coronary artery disease, as well as in neurological disorders. For example, sortilin has been identified as an important receptor for brain apolipoprotein E (APOE) metabolism, which is implicated in the underlying mechanisms of Alzheimer’s disease. A significant role for sortilin has recently also been reported in the field of oncology, as it has been detected in several cancer cell lines. Notably, human cancerous epithelial cells exhibited increased levels of sortilin as compared to normal epithelial tissues. Furthermore, it appears that sortilin participates in the progression of breast cancer and contributes to tumor cell adhesion and invasion.

Human SORT1(Sortilin) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of SORT1(Sortilin) The microtiter plate provided in this kit has been pre-coated with an antibody specific to SORT1(Sortilin). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SORT1(Sortilin). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain of SORT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SORT1(Sortilin) in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

1, Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

2, "Entrez Gene: SORT1 sortilin 1".

3, "BioGPS - your Gene Portal System". biogps.org. Retrieved 2016-08-16.

FAQ:

Q1. Is sample order available?
A: Yes, we welcome sample order to test and check quality.
Q2. What about the lead time?
A: Sample order: 3-5 days, mass order:10-12 days. The actual time depends on the order quantity.
Q3. Do you have any MOQ limit for led light order?
A: Low MOQ, 1pc for sample checking is available
Q4. How do you ship the goods and how many days will it take?
A: Our main shipping agent are DHL, FedEx, UPS and TNT, etc. It takes 3-7 days.
Q5. Is it OK to use my own package?
A: Yes. Normally we offer white inner box and carton box. Your own design is ok.

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