Wuhan Feiyue Biotechnology Co., LTD

Feiyue's Human SOD2(Superoxide dismutase [Mn], mitochondrial) ELISA Kit is an ELISA reagent for detection of SOD2(Alpha Crosslaps) in Mouse serum, plasma or cell with sensitivity, specificity and consistency.

Superoxide dismutase 2, mitochondrial (SOD2), also known as manganese-dependent superoxide dismutase (MnSOD), is an enzyme which in humans is encoded by the SOD2 gene on chromosome 6.[5][6] A related pseudogene has been identified on chromosome 1. Alternative splicing of this gene results in multiple transcript variants. This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. The SOD2 gene contains five exons interrupted by four introns, an uncharacteristic 5′-proximal promoter that possesses a GC-rich region in place of the TATA or CAAT, and an enhancer in the second intron. As a member of the iron/manganese superoxide dismutase family, this protein transforms toxic superoxide, a byproduct of the mitochondrial electron transport chain, into hydrogen peroxide and diatomic oxygen.[5] This function allows SOD2 to clear mitochondrial reactive oxygen species (ROS) and, as a result, confer protection against cell death. As a result, this protein plays an antiapoptotic role against oxidative stress, ionizing radiation, and inflammatory cytokines.

Human SOD2(Superoxide dismutase [Mn], mitochondrial) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of SOD2(Superoxide dismutase [Mn], mitochondrial) The microtiter plate provided in this kit has been pre-coated with an antibody specific to SOD2(Superoxide dismutase [Mn], mitochondrial). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SOD2(Superoxide dismutase [Mn], mitochondrial). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain of SOD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SOD2(Superoxide dismutase [Mn], mitochondrial) in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

1,"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

2, "Entrez Gene: SOD2 superoxide dismutase 2, mitochondrial".

3, Becuwe P, Ennen M, Klotz R, Barbieux C, Grandemange S (Dec 2014). "Manganese superoxide dismutase in breast cancer: from molecular mechanisms of gene regulation to biological and clinical significance". Free Radical Biology & Medicine. 77: 139–151.

FAQ:

Q1. Is sample order available?
A: Yes, we welcome sample order to test and check quality.
Q2. What about the lead time?
A: Sample order: 3-5 days, mass order:10-12 days. The actual time depends on the order quantity.
Q3. Do you have any MOQ limit for led light order?
A: Low MOQ, 1pc for sample checking is available
Q4. How do you ship the goods and how many days will it take?
A: Our main shipping agent are DHL, FedEx, UPS and TNT, etc. It takes 3-7 days.
Q5. Is it OK to use my own package?
A: Yes. Normally we offer white inner box and carton box. Your own design is ok.

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